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E.coli GT115大肠杆菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

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  • 货  号:E.coli GT115大肠杆菌菌株
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E.coli GT115大肠杆菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心                      

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E.coli GT115,SmR.


GT115 is a strain of E. coli that has been specifically engineered to support the growth of plasmid DNAs harboring the R6Kg origin of replication and carrying hairpin structures, such as pCpG-mcs, pCpG-LacZ and pCpG-siRNA vectors. GT115 has the pir gene that encodes the p protein which is required by vectors utilizing the R6Kg ori. Hairpin structures are known to be unstable in E. coli due to their elimination by a protein complex called SbcCD that recognizes and cleaves hairpins1 . To increase their stability in E. coli, we developed GT115 by deleting the sbcC and sbcD genes. This modification significantly improves the number of recombinant clones harboring a plasmid with hairpin structures. This strain contains the rpsL (StrA) gene which confers resistance to streptomycin.  GT115 Genotype:  F- mcrA ∆(mrr-hsdRMS-mcrBC) f80lacZDM15 ∆lacX74 recA1 rpsL (StrA) endA1 Ddcm uidA(DMluI)::pir-116 ∆sbcC-sbcD



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