SR cell line人胸膜渗出物大细胞免疫母细胞淋巴瘤细胞株-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2262

SR Human Pleural effusion Large Cell Immunoblastic Lymphoma CellLine

Cat No.: NTCC925434

Product category

Human cells

Organism

Homo sapiens, human

Cell type

lymphoblast

Morphology

lymphoblast

Tissue

Pleural effusion

Disease

Large Cell Immunoblastic Lymphoma

Applications

3D cell culture

Immunology

Characteristics

Growth properties

Suspension

Derivation

BioVector® SR is a human lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo.

Age

11 years

Ethnicity

White

Gender

Male

Antigen expression

Hle-1 +; HLA DQ +; HLA DR +; CD25 +; CD19 -; CD20 -; CD21 -; CD22 -; T cell receptor (TCR) -

Comments

BioVector® SR is of undetermined cellular origin because it expresses no markers unique to B, T, natural killer (NK) or monocyte-lineages.

Exposure of SR cells to protein kinase C activating phorbol esters such as PMA and PdBu do not induce growth inhibition.

BioVector® SR cells have been reported to be Epstein-Barr virus genome negative.

Handling information

Unpacking and storage instructions

1. Check all containers for leakage or breakage.

2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.

Complete medium

The base medium for this cell line is NTCC-formulated RPMI-1640 Medium, NTCC30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (NTCC30-2020) to a final concentration of 10%.

Handling procedure

To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.

1. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).

2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.

3. Transfer the vial contents to a 75 cm2 tissue culture flask and dilute with the recommended complete culture medium (see the specific batch information for the recommended dilution ratio). It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).

4. Incubate the culture at 37°C in a suitable incubator.  A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.

Note: If it is desired that the cryoprotective agent be removed immediately, or that a more concentrated cell suspension be obtained, centrifuge the cell suspension at approximately 125 x g for 5 to 10 minutes.  Discard the supernatant and resuspend the cells with fresh growth medium at the dilution ratio recommended in the specific batch information.

Subculturing procedure

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 - 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL.

Medium Renewal: 2 to 3 times a week.

Reagents for cryopreservation

Complete growth medium supplemented with 5% (v/v) DMSO

Quality control specifications

Mycoplasma contamination

Not detected

SR cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2262
【Organism物种来源】人源Homo sapiens, human  
【Tissue组织来源】
【Cell Type细胞类型】  
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】  
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】 ­
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doubling Time倍增殖时间】
【References参考文献】

【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net