pAD123枯草芽孢杆菌表达载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

基本信息
复制子: ori
质粒大小: 5952bp
质粒标签: GFP
原核抗性: Amp
克隆菌株: DH5α
培养条件: 37℃，有氧 LB
表达宿主: 枯草芽孢杆菌
诱导方式: IPTG诱导
5'测序引物: 根据序列设计引物
3'测序引物: 根据序列设计引物
质粒简介
   We constructed a promoter-trap plasmid, pAD123, for Bacillus cereus. This plasmid contains a promoterless gene that encodesa mutant version of the green fluorescent protein, GFPmut3a, that is optimized for fluorescence-activated cell sorting [Cormack, FACS-optimized mutants of the green fluorescent protein (GFP).

质粒图谱
质粒序列
LOCUS       Exported File           5952 bp ds-DNA    circular SYN 29-5-2015
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    Untitled
SOURCE      synthetic DNA construct
 ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5952)
 AUTHORS   .
 TITLE     Direct Submission
 JOURNAL   Exported 2015-5-29 from
           http://www.biovector.net
FEATURES             Location/Qualifiers
    source          1..5952
                    /organism="synthetic DNA construct"
                    /mol_type="other DNA"
    CDS             54..770
                    /codon_start=1
                    /product="Aequoria victoria green fluorescent protein"
                    /note="GFP"
                    /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                    FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                    NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                    NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                    FVTAAGITHGMDELYK"
    misc_feature    3802..3942
                    /note="bom"
                    /note="basis of mobility region from pBR322"
    rep_origin      complement(4128..4716)
                    /direction=LEFT
                    /note="ori"
                    /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                    replication"
    CDS             complement(4887..5747)
                    /codon_start=1
                    /gene="bla"
                    /product="beta-lactamase"
                    /note="AmpR"
                    /note="confers resistance to ampicillin, carbenicillin, and
                    related antibiotics"
                    /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                    ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                    PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                    EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                    LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                    LIKHW"
    promoter        complement(5748..5852)
                    /gene="bla"
                    /note="AmpR promoter"
ORIGIN
       1 gaattcgagc tcggtacccg gggatcctct agatttaaga aggagatata catatgagta
      61 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
     121 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
     181 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
     241 ctttcgggta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
     301 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
     361 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
     421 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
     481 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
     541 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
     601 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
     661 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
     721 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa atctcctgca
     781 ggcatgcaag cttgagtagg acaaatccgc cgagcttcga cgagattttc aggagctaag
     841 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     901 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     961 cagaacaaag aatacaagaa aatatttaca aaaaatcaat ttaacaattc cttaaaacat
    1021 gcaggaattg acgatttaaa caatattagc tttgaacaat tcttatctct tttcaatagc
    1081 tataaattat ttaataagta agttaaggga tgcataaact gcatccctta acttgttttt
    1141 cgtgtgccta ttttttgtga atcgctaaga aaccattatt atcatgacat taacctataa
    1201 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    1261 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    1321 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    1381 ggcatcagag cagattgtac tgagagtgca ccatacaaaa catatttcaa cacaatacaa
    1441 atgggttagt taaaaaagca ggccttctaa aggtctgctt tttttatttg attatgtaat
    1501 ttttaatgcc aggatgccaa taagccataa cctcaaatgc accatttgca acctcgtcat
    1561 cttcttcctc aatcttgacc agatcgccgt cctccgcatc accaagattc agctctttat
    1621 gtatctcctt caaaatgcca ccgtatccaa ttaaccttcg agctgccaac gcatcatcca
    1681 agtaaagcac cgtgttcaga ttgtcttcag tcaccttatt accgcgcaca caatccgtat
    1741 ccttaaccgg atatttagag atttcgcgaa cagctttttg ctccatcatt gcgttccgca
    1801 catcgttttc aatctgttca gcgtcaatct tagctttacc tttcactcga cgaatatcga
    1861 caattggagt gtaatccaat ttcatcgcct ttttccaaag gctcgtccac tccgcctgct
    1921 taatatagtt tttcccaaaa taatttttcc ttactggtat caacacatga aaatgaggat
    1981 gatatgtatc ttcttcatga tttttggtaa tctctaaagc tctgaaaaat ccaagaaccg
    2041 aagtttttac ttttttgtac tggaacagtt tcctaaagcc ttccatcatc gcagaaattt
    2101 gtggcttcag ccgttctccc tttacatttc gaatcgtcag cgtgagaaaa atccatccgc
    2161 agccgtactg tctattggct tcctctacga tcaacttatt gtgataagca atttttaacg
    2221 acctgcgcca cgcacacatc ggacataacc tcactttaca aaaatgggct tgatacagtt
    2281 ttaacttgcc cgtctccggg tctctcttaa acgaaagata ctctgcacaa ctaattagtt
    2341 tttcagcctt tttgccatag taaggtgccc caatcttact ctctaacgct tcgtaatgct
    2401 ccgccatgag gttcgtccgt ctctttttcc ccttccaatc ccgcttttta cctgttgcgg
    2461 ttttatcttc gaggatgcta taatcatttt cagatgaata aatcaacaaa aaaactcctt
    2521 ctgagctagt tctctagcat tctattattt tgattcgaca ccttaataat agcagaagga
    2581 gtttttacct gtcaaagaac catcaaaccc ttgatacaca aggctttgac ctaattttga
    2641 aaaatgatgt tgtttctata tagtatcaag ataagaaaga aaaggatttt tcgctacgct
    2701 caaatccttt aaaaaaacac aaaagaccac attttttaat gtggtcttta ttcttcaact
    2761 aaagcaccca ttagttcaac aaacgaaaat tggataaagt gggatatttt taaaatatat
    2821 atttatgtta cagtaatatt gacttttaaa aaaggattga ttctaatgaa gaaagcagac
    2881 aagtaagcct cctaaattca ctttagataa aaatttagga ggcatatcaa atgaacttta
    2941 ataaaattga tttagacaat tggaagagaa aagagatatt taatcattat ttgaaccaac
    3001 aaacgacttt tagtataacc acagaaattg atattagtgt tttataccga aacataaaac
    3061 aagaaggata taaattttac cctgcattta ttttcttagt gacaagggtg ataaactcaa
    3121 atacagcttt tagaactggt tacaatagcg acggagagtt aggttattgg gataagttag
    3181 agccacttta tacaattttt gatggtgtat ctaaaacatt ctctggtatt tggactcctg
    3241 taaagaatga cttcaaagag ttttatgatt tatacctttc tgatgtagag aaatataatg
    3301 gttcggggaa attgtttccc aaaacaccta tacctgaaaa tgctttttct ctttctatta
    3361 ttccatggac ttcatttact gggtttaact taaatatcaa taataatagt aattaccttc
    3421 tacccattat tacagcagga aaattcatta ataaaggtaa ttcaatatat ttaccgctat
    3481 ctttacaggt acatcattct gtttgtgatg gttatcatgc aggattgttt atgaactcta
    3541 ttcaggaatt gtcagatagg cctaatgact ggcttttata atatgagata atgccgactg
    3601 tactttttac agtcggtttt ctaatgtcac taacctgccc cgttagtcgc cattcgccag
    3661 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    3721 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    3781 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
    3841 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    3901 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
    3961 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    4021 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    4081 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    4141 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    4201 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    4261 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    4321 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    4381 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    4441 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    4501 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    4561 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    4621 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    4681 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    4741 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    4801 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    4861 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    4921 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    4981 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    5041 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    5101 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    5161 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
    5221 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    5281 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    5341 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    5401 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    5461 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
    5521 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    5581 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    5641 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    5701 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    5761 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    5821 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    5881 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    5941 tttcgtcttc aa
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