Thialkalivibrio versutus No.:13738 BioVector NTCC质粒载体菌种细胞基因保藏中心

Thialkalivibrio versutus
Cat No.:13738

BioVector NTCC质粒载体菌种细胞基因保藏中心                      

Name:
Thioalkalivibrio versutus Sorokin et al. 2001
Synonym(s):
Thialkalivibrio versutus corrig. Sorokin et al. 2001
DSM No.:
13738, Type strain
Strain designation:
AL2
Other collection no.
or WDCM no.:
CBS 100464
Isolated from:
soda lake
Geographic origin:
Russia
History:
<- D. Y. Sorokin; AL2
Genbank accession numbers:
16S rRNA gene: AF126546
16S rRNA gene: FR749906
Cultivation conditions:
Medium 925 , 30°C
Summary and
additional information:
<- D. Y. Sorokin; AL2. (Thialkalivibrio versutus). Soda lake; Russia.Type strain. Taxonomy/description (8049). Sequence accession no. 16S rRNA gene: AF126546 (this sequence may contain a number of errors, D.Y. Sorokin pers. comm.), 16S rRNA gene: FR749906. (Medium 925, 30°C).
For detailed information:
BacDive - The Bacterial Diversity Metadatabase
Literature:
Sorokin, D. Y., Lysenko, A. M., Mityushina, L. L., Tourova, T. P., Jones, B. E., Rainey, F. A., Robertson, L. A., Kuenen, G. J. (2001). Thioalkalimicrobium aerophilum gen. nov., sp. nov. and Thioalkalimicrobium sibericum sp. nov., and Thioalkalivibrio versutus gen. nov., sp. nov.,Thioalkalivibrio nitratis sp. nov. and Thioalkalivibrio denitrificans sp. nov., novel obligately alkaliphilic and obligately chemolithoautotrophic sulfur-oxidizing bacteria from soda lakes.Int.J.Syst.Evol.Microbiol. 51 : 565-580 . http://www.ncbi.nlm.nih.gov/pubmed/11321103
Risk group:
1

925. ALKALIPHILIC SULPHUR RESPIRING STRAINS MEDIUM
Mineral base:
Na2CO3 20.0 g
NaHCO3 10.0 g
NaCl 5.0 g
K2HPO4 1.0 g
Distilled water 1000.0 ml
Sterilize at 110C 20 min in a closed vessel (i.e. a serum tube or bottle). pH after
sterilization will about 10.
Trace element solution:
EDTA 5.0 mg
FeSO4 x 7 H2O 2.0 mg
ZnSO4 x 7 H2O 100.0 mg
MnCl2 x 4 H2O 30.0 mg
CoCl2 x 6 H2O 200.0 mg
NiCl2 x 6 H2O 20.0 mg
Na2MoO4 x 2 H2O 30.0 mg
CuCl2 x 2 H2O 10.0 mg
H3BO3 300.0 mg
Distilled water 1000.0 ml
Final pH should be 3, add HCl if needed. Sterilization - 120C 20 min.
After sterilization add:
Trace elements solution 2 ml/l
MgCl2 x 6 H2O (200.0 g/l) 1 ml/l
(a white colloid will form which will rapidly dissolve after mixing)
Prepare sterile stock solutions of the following
Sodium thiosulfate pentahydrate (2 M - 496 g/l)
KSCN solution (2 M - 194 g/l)
NH4Cl - (1 M - 53.5 g/l)
KNO3 - (1 M - 101 g/l)
Growth:
DSM 13531 = ARh1 (KSCN grown):
Growth with thiocyanate : add KSCN up to 15 mM, incubation - 30C in conical flasks
1/10 liquid to air ratio, statically;
growth is slow, about 1 week
DSM 13541 = ARh2 (thiosulphate grown):
Growth with thiosulfate: add 40 mM thiosulfate and 5 mM NH4Cl, incubate in closed
flasks, 1/10 liquid to air on shaker 200 rpm; heavy sulfur formation is usual, it is
necessary to wait until all the sulfur is consumed (about 1 week).  DSM 13532 = ARh2 (KSCN grown):
Growth with 15 mM KSCN: same conditions as for DSM 13531 = ARh1.
DSM 13542 = ARh1 (thiosulphate grown)
Growth with thiosulfate (40 mM) and nitrate (10 mM); in conical flasks 1/5 liquid/air
ratio on shaker 200 rpm. Growth takes 3-4 days, some sulfur can be formed during
initial growth phase.
DSM 13533 = ALRh
strains ALRh: grows with 40 mM thiosulfate and 5 mM KSCN in
conical flasks 1/5 liquid to air ratio at 200 rpm.
Thioalkalivibrio versutus AL 2 = DSM 13738, Tv.nitratus ALJ 12 = DSM 13741, and
Tv.denitrificans ALJD = DSM 13742
Use 40 mM thiosulphate, 5 mM KNO3, reduce the amount of MgCl2 to 0.5 mM
Thioalkalimicrobium aerophilum AL 3 = DSM 13739 and Tm.sibericum AL 7 = DSM 13740
Use 80 mM thiosulphate, 5 mM KNO3, reduce the amount of MgCl2 to 0.5 mM
Thioalkalispira microaerophila, ALEN 1 = DSM 14786. The strain is grown under nitrogen
with 1% oxygen in the gas phase. The strain grows slowly with a potential lag phase of
several days.
Use 30 mM thiosulphate and up to a maximum of 5 mM NH4Cl
Thioalkalivibrio nitratireducens ALEN 2 = DSM 14787
Use 30 - 40 mM thiosulphate and 5-10 mM KNO3
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