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MSCV-IRES-GFP 逆病毒载体质粒图谱序列抗性

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  • 货  号:3558669
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MSCV-IRES-GFP 
(BioVector-020672)

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Available to Academic and Nonprofits Only

BACKBONE

  • Vector backbone
    MSCV
  • Backbone size (bp)5900
  • Vector type
    Mammalian Expression, Retroviral
  • Tag / Fusion Protein
    • IRES EGFP (C terminal on backbone)

GROWTH IN BACTERIA

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    High Copy

SEQUENCE INFORMATION

  • Depositor Sequences
    None.
  • Addgene Sequences

Full plasmid sequence is available only if provided by the depositing laboratory.

CLONING INFORMATION

  • Cloning methodRestriction Enzyme
  • 5′ sequencing primerpLXSN5; pBABE5
  • 3′ sequencing primerpCDH-rev (GCATTCCTTTGGCGAGAG)
  • (Common Sequencing Primers)

RESOURCE INFORMATION

DEPOSITOR COMMENTS

This plasmid is essentially pMIG and we suggest ordering it instead of plasmid 9044 as it has better yield in culture and is easier to sequence.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-IRES-GFP was a gift from Tannishtha Reya (Addgene plasmid # 20672)

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