- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
Plasmid: pMXs
Analyze:
Sequence
Plasmid Type:
Mammalian Expression, Retroviral
Cloning Method:
Unknown
5 Sequencing 1 Primer:
GAC GGC ATC GCA GCT TGG ATA CAC (you can also use pBMN5)
Bacterial Resistance:
Ampicillin
Notes:
Described in Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Unlike most other retrovirus vectors such as LXSN and MSCV, the pMX vector harbors the splicing donor and acceptor sites and produces two types of transcripts, the full-length genome RNA and the subgenomic RNA. Therefore, in addition to the correct proteins produced from the subgenomic RNA, Gag-fusion proteins could be expressed from the full-length genome RNA if the reading frame of gag and that of the inserted gene match and there is no stop codon between them. To generate improved vectors free of the Gag and the Gag-fusion proteins, we disrupted the ATG start codon of gag, inserted a stop codon downstream of the CTG start codon of glyco gag, and inserted triple stop codons in three different reading frames just before MCS. The improved vectors are termed pMXs. A variety of pMXs series are depicted in the included map, including pMXs-puro, pMXs-neo, pMXs-IG (IRES-GFP), pMXs-IN (IRES-neo), and pMXs-IP (IRES-puro). pMYs, pMZs, and pMCs are also described in this publication.
Stable:
Unspecified
Constitutive:
Unspecified
Viral/Non-Viral:
Retroviral
| Analyze: | Sequence |
| Plasmid Type: | Mammalian Expression, Retroviral |
| Cloning Method: | Unknown |
| 5 Sequencing 1 Primer: | GAC GGC ATC GCA GCT TGG ATA CAC (you can also use pBMN5) |
| Bacterial Resistance: | Ampicillin |
| Notes: |
Described in Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Unlike most other retrovirus vectors such as LXSN and MSCV, the pMX vector harbors the splicing donor and acceptor sites and produces two types of transcripts, the full-length genome RNA and the subgenomic RNA. Therefore, in addition to the correct proteins produced from the subgenomic RNA, Gag-fusion proteins could be expressed from the full-length genome RNA if the reading frame of gag and that of the inserted gene match and there is no stop codon between them. To generate improved vectors free of the Gag and the Gag-fusion proteins, we disrupted the ATG start codon of gag, inserted a stop codon downstream of the CTG start codon of glyco gag, and inserted triple stop codons in three different reading frames just before MCS. The improved vectors are termed pMXs. A variety of pMXs series are depicted in the included map, including pMXs-puro, pMXs-neo, pMXs-IG (IRES-GFP), pMXs-IN (IRES-neo), and pMXs-IP (IRES-puro). pMYs, pMZs, and pMCs are also described in this publication.
|
| Stable: | Unspecified |
| Constitutive: | Unspecified |
| Viral/Non-Viral: | Retroviral |
Generated Plasmid Map
关于我们 下单订购 加入我们 Copyright © 2023 京ICP备17017111号-1 京ICP备13016347号-3 BioVector质粒载体菌种细胞蛋白抗体基因保藏中心-NTCC典型培养物保藏中心 - All Rights Reserved.
扫码支付: 名片码:
免费订购电话: 400-800-2947
北京实验室: 189-01268599
订单及咨询邮箱：Biovector@163.com
企业QQ/微信：1843439339
经销商联系：189-01268599