pCMV-myc质粒载体图谱序列抗性说明书价格报价

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产品分类

原核表达载体

pCMVmyc载体基本信息

出品公司:载体名称:质粒类型:高拷贝/低拷贝:启动子:克隆方法:载体大小:5' 测序引物及序列:3' 测序引物及序列:载体标签:载体抗性:筛选标记:备注:产品目录号:稳定性:组成型:病毒/非病毒:

Clontech

pCMV-myc

哺乳细胞表达系统载体

CMV

多克隆位点，限制性内切酶

3791bp

pCMV：GATCCGGTACTAGAGGAACTGAAAAAC

Myc (N-term) (MASMQKLISEEDL)

Ampicillin

用于哺乳细胞表达N端带有Mcy标签的蛋白

631604

瞬表达

组成型

非病毒

pCMVmyc载体质粒图谱和多克隆位点信息

pCMV-myc载体图谱

pCMVmyc载体简介

pCMVmyc载体序列

LOCUS pCMV-Myc 3791 bp DNA SYN
DEFINITION pCMV-Myc
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3791
/organism="pCMV-Myc"
/mol_type="other DNA"
misc_feature 31..318
/label="CAG_enhancer"
promoter 36..528
/label="CMV_immearly_promoter"
misc_feature 485..505
/label="CMV_fwd_primer"
promoter 486..555
/label="CMV_promoter"
promoter 498..617
/label="CMV2_promoter"
misc_feature 529..548
/label="pCEP_fwd_primer"
misc_feature 531..555
/label="LNCX_primer"
misc_feature 631..657
/label="pCMV_primer"
misc_feature 672..768
/label="modSV40_late_16s_int"
misc_feature 672..702
/label="SV40_late_19s_int"
misc_feature 832..855
/label="Myc_primer"
terminator complement(940..1059)
/label="SV40_PA_terminator"
misc_feature complement(954..973)
/label="EBV_rev_primer"
promoter complement(1171..1189)
/label="M13_reverse_primer"
misc_feature complement(1188..1210)
/label="M13_pUC_rev_primer"
promoter complement(1224..1253)
/label="lac_promoter"
gene complement(2336..3196)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(2336..3196)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(3238..3266)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature complement(3425..3447)
/label="pGEX_3_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature complement(3646..3789)
/label="lacZ_a"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 3760..3782
/label="M13_pUC_fwd_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 3775..3791
/label="M13_forward20_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSD