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pET-11c(+)图谱序列抗性价格Biovector质粒库

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pet11c载体基本信息

出品公司:别名:质粒类型:表达水平:克隆方法:载体大小:5' 测序引物:5' 测序引物序列:  载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:
EMD Biosciences(Novagen)
pET11c, pet 11c
大肠杆菌蛋白表达
多克隆位点,限制性内切酶
5672bp
T7
5'-TAATACGACTCACTATAGGG-3'
N-T7
Ampicillin
Approximate size is 5675 bp. Hosts: E.coli. Related vectors: pBR322.
69438-3
瞬时表达 Transient
组成型 Constitutive
非病毒

pet11c载体质粒图谱和多克隆位点信息

pet11c载体图谱
pet11c载体多克隆位点

Feature Name
Start
End
 
T7_terminator316188
T7_Terminal_primer256274
T7_leader358326
Xpress_fwd_primer359341
T7_transl_en_RBS383367
lacO428401
T7_promoter446428
pBRrevBam_primer549530
lacI8241915
tet (576 - 1190)19742588
ROP32273418
pGEX_3_primer34343412
Ampicillin54644604
AmpR_promoter55345506
ORF
Start
End
 
ORF frame 15831083
ORF frame 29561915
ORF frame 123931698
ORF frame 319712588
ORF frame 254644604
Enzyme Name
Cut
ClaI24
HindIII29
BamHI319
NheI350
NdeI357
XbaI395
BglII461
BclI1197
ApaI1394
HpaI1689
EagI2251
NruI2286
MscI2758
PstI4922
AatII5599
EcoRI5670

pet11c载体简介

The pET-11a-d vectors carry an N-terminal T7•Tag® sequence and BamH I cloning site. Thesevectors are the precursors to many pET family vectors; the pET-21a-d(+) series corresponds topET-11a-d but incorporates several additional features. Unique sites are shown on the circle map.Note that the sequence is numbered by the pBR322 convention, so the T7 expression region isreversed on the circular map. The cloning/expression region of the coding strand transcribed byT7 RNA polymerase is shown below.

pet11c载体序列


    1 TTCTCATGTT TGACAGCTTA TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA
   61 TTGCTAACGC AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG
  121 CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC CGGGCCTCTT
  181 GCGGGATATC CGGATATAGT TCCTCCTTTC AGCAAAAAAC CCCTCAAGAC CCGTTTAGAG
  241 GCCCCAAGGG GTTATGCTAG TTATTGCTCA GCGGTGGCAG CAGCCAACTC AGCTTCCTTT
  301 CGGGCTTTGT TAGCAGCCGG ATCCGACCCA TTTGCTGTCC ACCAGTCATG CTAGCCATAT
  361 GTATATCTCC TTCTTAAAGT TAAACAAAAT TATTTCTAGA GGGGAATTGT TATCCGCTCA
  421 CAATTCCCCT ATAGTGAGTC GTATTAATTT CGCGGGATCG AGATCTCGAT CCTCTACGCC
  481 GGACGCATCG TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC
  541 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC
  601 GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCATGCA
  661 CCATTCCTTG CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG
  721 CAGGAGTCGC ATAAGGGAGA GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT
  781 TCGCGGTATG GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC
  841 AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT
  901 GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC
  961 GGAGCTGAAT TACATTCCCA ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT
 1021 GATTGGCGTT GCCACCTCCA GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT
 1081 TAAATCTCGC GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG
 1141 CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT
 1201 CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT
 1261 TCCGGCGTTA TTTCTTGATG TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCA
 1321 TGAAGACGGT ACGCGACTGG GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC
 1381 GCTGTTAGCG GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA
 1441 ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT
 1501 GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGAGGGC ATCGTTCCCA CTGCGATGCT
 1561 GGTTGCCAAC GATCAGATGG CGCTGGGCGC AATGCGCGCC ATTACCGAGT CCGGGCTGCG
 1621 CGTTGGTGCG GATATCTCGG TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT
 1681 CCCGCCGTTA ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG
 1741 CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC CCGTCTCACT
 1801 GGTGAAAAGA AAAACCACCC TGGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC
 1861 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA
 1921 ACGCAATTAA TGTAAGTTAG CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA
 1981 GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC
 2041 TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTGGGTCA
 2101 TTTTCGGCGA GGACCGCTTT CGCTGGAGCG CGACGATGAT CGGCCTGTCG CTTGCGGTAT
 2161 TCGGAATCTT GCACGCCCTC GCTCAAGCCT TCGTCACTGG TCCCGCCACC AAACGTTTCG
 2221 GCGAGAAGCA GGCCATTATC GCCGGCATGG CGGCCGACGC GCTGGGCTAC GTCTTGCTGG

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